e fs e073  (Elabscience Biotechnology)

Journal: Molecules

Article Title: Upregulated Palmitoleate and Oleate Production in Escherichia coli Promotes Gentamicin Resistance

doi: 10.3390/molecules29112504

Figure Lengend Snippet: Changes in the metabolic profiles of gentamicin-resistant E. coli . ( A ) The MIC of gentamicin-resistant E. coli . ( B ) The growth curve of gentamicin-resistant E. coli . n = 4 per group. *, p < 0.05, **, p < 0.01, two-tailed independent samples t -test. ( C ) Changes in the metabolic profiles of gentamicin-resistant E. coli . S, gentamicin-sensitive E. coli ; R, gentamicin-resistant E. coli . n = 6, 6 and 3 in the S, R and QC group, respectively. ( D ) The reproducibility and stability of detected ions in QC samples.

Article Snippet: Following centrifugation at 12,000× g for 10 min at 4 °C, cells were collected for detecting intracellular gentamicin contents according to the ELISA kit (CUSABIO, Wuhan, Hubei, China).

Techniques: Two Tailed Test

Journal: Molecules

Article Title: Upregulated Palmitoleate and Oleate Production in Escherichia coli Promotes Gentamicin Resistance

doi: 10.3390/molecules29112504

Figure Lengend Snippet: Metabolic reprogramming in gentamicin-resistant E. coli . ( A ) Heat map plot of metabolic reprograming in gentamicin-resistant E. coli . After data normalization to unit variance, the metabolite level was employed for the heat map plot. n = 6 per group. Metabolites significantly altered in gentamicin-resistant E. coli compared to the control ( p < 0.05, two-tailed Mann–Whitney U test) are all shown in the plot. S, gentamicin-sensitive E. coli ; R, gentamicin-resistant E. coli . ( B ) Changes in metabolic pathways. All pathways significantly altered in gentamicin-resistant E. coli compared to the control ( p < 0.05) are shown.

Article Snippet: Following centrifugation at 12,000× g for 10 min at 4 °C, cells were collected for detecting intracellular gentamicin contents according to the ELISA kit (CUSABIO, Wuhan, Hubei, China).

Techniques: Control, Two Tailed Test, MANN-WHITNEY

Journal: Molecules

Article Title: Upregulated Palmitoleate and Oleate Production in Escherichia coli Promotes Gentamicin Resistance

doi: 10.3390/molecules29112504

Figure Lengend Snippet: Reprogramming of carbohydrate ( A ) and nucleotide ( B ) metabolism in gentamicin-resistant E. coli . S, gentamicin-sensitive E. coli ; R, gentamicin-resistant E. coli . n = 6 per group. *, p < 0.05, **, p < 0.01, two-tailed Mann–Whitney U test.

Article Snippet: Following centrifugation at 12,000× g for 10 min at 4 °C, cells were collected for detecting intracellular gentamicin contents according to the ELISA kit (CUSABIO, Wuhan, Hubei, China).

Techniques: Two Tailed Test, MANN-WHITNEY

Journal: Molecules

Article Title: Upregulated Palmitoleate and Oleate Production in Escherichia coli Promotes Gentamicin Resistance

doi: 10.3390/molecules29112504

Figure Lengend Snippet: Reprogramming of amino acid metabolism in gentamicin-resistant E. coli . S, gentamicin-sensitive E. coli ; R, gentamicin-resistant E. coli . n = 6 per group. *, p < 0.05, **, p < 0.01, two-tailed Mann–Whitney U test. Blue fonts: metabolites significantly decreased in gentamicin-resistant E. coli , which are shown in .

Article Snippet: Following centrifugation at 12,000× g for 10 min at 4 °C, cells were collected for detecting intracellular gentamicin contents according to the ELISA kit (CUSABIO, Wuhan, Hubei, China).

Techniques: Two Tailed Test, MANN-WHITNEY

Journal: Molecules

Article Title: Upregulated Palmitoleate and Oleate Production in Escherichia coli Promotes Gentamicin Resistance

doi: 10.3390/molecules29112504

Figure Lengend Snippet: Reprogramming of lipid metabolism in gentamicin-resistant E. coli . S, gentamicin-sensitive E. coli ; R, gentamicin-resistant E. coli ; MG, monoglyceride. n = 6 per group. **, p < 0.01, two-tailed Mann–Whitney U test.

Article Snippet: Following centrifugation at 12,000× g for 10 min at 4 °C, cells were collected for detecting intracellular gentamicin contents according to the ELISA kit (CUSABIO, Wuhan, Hubei, China).

Techniques: Two Tailed Test, MANN-WHITNEY

Journal: Molecules

Article Title: Upregulated Palmitoleate and Oleate Production in Escherichia coli Promotes Gentamicin Resistance

doi: 10.3390/molecules29112504

Figure Lengend Snippet: Potential biomarkers related to gentamicin resistance in E. coli . ( A ) Volcano plot of metabolic changes in gentamicin-resistant E. coli . Only the significantly altered metabolites are shown ( p < 0.05, two-tailed Mann–Whitney U test). ( B ) The diagnostic performances of the top 6 metabolites with the largest increases in the evaluation of gentamicin resistance in E. coli . The values of area under the curve (AUC) and related confidence interval (CI) were calculated by performing a classical univariate receiver operating characteristic curve analysis. The accuracy rate was calculated from the sample discrimination result using the corresponding potential biomarker via the support vector machine algorithm. ( C ) The potential biomarker for evaluating gentamicin resistance in E. coli . Metabolites were used separately in the model of support vector machines for identification of gentamicin resistance. S, gentamicin-sensitive E. coli ; R, gentamicin-resistant E. coli . n = 6 per group.

Article Snippet: Following centrifugation at 12,000× g for 10 min at 4 °C, cells were collected for detecting intracellular gentamicin contents according to the ELISA kit (CUSABIO, Wuhan, Hubei, China).

Techniques: Two Tailed Test, MANN-WHITNEY, Diagnostic Assay, Biomarker Discovery, Plasmid Preparation

Journal: Molecules

Article Title: Upregulated Palmitoleate and Oleate Production in Escherichia coli Promotes Gentamicin Resistance

doi: 10.3390/molecules29112504

Figure Lengend Snippet: Effects of palmitoleate and oleate on gentamicin resistance in E. coli . Effects of palmitoleate ( A ) or oleate ( B ) on the survival rate of gentamicin-sensitive E. coli , the MIC of gentamicin ( C ) and intracellular contents of gentamicin ( D ) and ROS ( E ). The column represents the mean + standard deviation. n = 3 per group. *, p < 0.05, **, p < 0.01, two-tailed independent samples t -test.

Article Snippet: Following centrifugation at 12,000× g for 10 min at 4 °C, cells were collected for detecting intracellular gentamicin contents according to the ELISA kit (CUSABIO, Wuhan, Hubei, China).

Techniques: Standard Deviation, Two Tailed Test